TABLE 1.

Primers used in this study

Primer	Sequence (5′ → 3′)	Usage
HA-ZAP-F	CCGGAATTCGCCACCATGGCGGACCCGGAGGTGTG	Amplification of ZAP and ZAP-L
HA-ZAP-R	CGGGGTACCTCAAGCGTAATCTGGAACATCGTATGGGTACTCTGGCCCTCTCTTCATC	Amplification of ZAP and ZAP-M
HA-ZAP-L-R	CGGGGTACCTCAAGCGTAATCTGGAACATCGTATGGGTAACTAATCACGCAGGCTTTG	Amplification of ZAP-L and ZAP-C
HA-ZAP-N-R	CGGGGTACCTCACTTATCGTCGTCATCCTTGTAATCGTTTCTACGATGTGAAGA	Amplification of ZAP-N
HA-ZAP-M-F	CCGGAATTCGCCACCATGGCGTATAGGGCTAGA	Amplification of ZAP-M
HA-ZAP-C-F	CCGGAATTCGCCACCATGCGTCAGCCAGCACAGACCTCG	Amplification of ZAP-C
Flag-Nsp9-F	CGGGGTACCGCCACCATGGATTACAAGGATGACGACGATAAGTTTAAACTGCTAGCCG	Amplification of Nsp9 and 1–177/449
Flag-Nsp9-R	CCGCTCGAGTCACTCATGATTGGACCTG	Amplification of Nsp9 and 60/119/150/160/170–643
Flag-Nsp9 177-R	CCGCTCGAGTCAGCTTCCAGTGTCACTG	Amplification of Nsp9 1–177
Flag-Nsp9 449-R	CCGCTCGAGTCAGCCGCCTCTCTTAGTCAC	Amplification of Nsp9 1–449
Flag-Nsp9 178-F	CGGGGTACCGCCACCATGGATTACAAGGATGACGACGATAAGCCGGTGCATGCAGCTG	Amplification of Nsp9 178–449/643
Flag-Nsp9 450-F	CGGGGTACCGCCACCATGGATTACAAGGATGACGACGATAAGCTTTCGTCTGGCGACCCG	Amplification of Nsp9 450–643
Flag-Nsp9 60-F	CGGGGTACCGCCACCATGGATTACAAGGATGACGACGATAAGGTTGCCCGACCGGTTGA	Amplification of Nsp9 60–643
Flag-Nsp9 119-F	CGGGGTACCGCCACCATGGATTACAAGGATGACGACGATAAGGTTGCACTCAGTGCGCA	Amplification of Nsp9 119–643
Flag-Nsp9 150-F	CGGGGTACCGCCACCATGGATTACAAGGATGACGACGATAAGGGCAACCCTGAGCGGGT	Amplification of Nsp9 150–643
Flag-Nsp9 160-F	CGGGGTACCGCCACCATGGATTACAAGGATGACGACGATAAGCAGAATACAAGGTTTG	Amplification of Nsp9 160–643
Flag-Nsp9 170-F	CGGGGTACCGCCACCATGGATTACAAGGATGACGACGATAAGAAAACCCCCAGTGACAC	Amplification of Nsp9 170–643
Q-PRRSV-N-F	AAACCAGTCCAGAGGCAAGG	qRT-PCR for detection of PRRSV
Q-PRRSV-N-R	TCAGTCGCAAGAGGGAAATG
Q-ZAP-F	CCACATCTTCTAGGGTGGATGA	qRT-PCR for detection of ZAP
Q-ZAP-R	CGTCCAGGTTTCACCAATAAACA
Q-GAPDH-F	GAAGGTGAAGGTCGGAGTC	qRT-PCR for detection of GAPDHa
Q-GAPDH-R	GAAGATGGTGATGGGATTTC

^aGAPDH, glyceraldehyde-3-phosphate dehydrogenase.