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. 2019 May 1;93(10):e01801-18. doi: 10.1128/JVI.01801-18

FIG 3.

FIG 3

(A) Illustration of E2 truncations and deletions. HEK293TT cells were transfected with FLAG-FGFR3 K650E and these FLAG-HPV-31 E2 constructs. (B) After 48 h, cells expressing aa 107 to 372 and aa 176 to 372 were treated with 20 μM MG132 for 6 h. Cells were lysed, and FLAG-FGFR3 K650E immunoprecipitated with FGFR3 antibodies. Complexes were blotted with FLAG antibodies. (C) FLAG-HPV-31 E2 and FLAG-FGFR3 K650E immunoprecipitated with FLAG antibodies. Complexes were blotted with PY-1000 and FLAG antibodies (D) FLAG-FGFR3 K650E was immunoprecipitated with FGFR3 antibodies. Complexes were blotted with FLAG antibodies. (E) FLAG-HPV-31 E2 and FLAG-FGFR3 K650E immunoprecipitated with FLAG antibodies. Complexes were blotted with PY-1000 and FLAG antibodies.