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E2 Y138 mutants are nuclear, but Y138E do not form replication foci. CV-1 cells were transfected with FLAG-HPV-31 E2 Y138 mutants, HA-HPV-31 E1, and HPV-31 ori. Immunofluorescence staining was carried out with FLAG (red) and E1 (green) antibodies at 100×; 4′,6-diamidino-2-phenylindole (DAPI; blue) was used to stain the nuclei.
