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Y138E binds HPV-31 E1 and GPS-2. (A) HEK293TT cells were transfected with HA-HPV-31 E1 and FLAG-HPV-31 E2 Y138 mutants. FLAG pulldown was completed with M2 gel and immunoblotted with 16E1 and FLAG antibodies. Densitometry for four independent experiments is shown. E1 levels were normalized to E2 levels. (B) HEK293TT cells were transfected with HA-GPS2 and FLAG-HPV-31 E2 Y138 mutants. FLAG pulldown was completed with M2 gel and immunoblotted with GPS-2, HPV-16 E1, and FLAG antibodies.
