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. 2019 May 1;93(10):e01801-18. doi: 10.1128/JVI.01801-18

FIG 7.

FIG 7

Y138E does not bind to the Brd4 C-terminal domain (CTD). (A) HEK293TT cells were transfected with FLAG-Brd4 full-length and FLAG-HPV-31 E2 Y138 mutants. Brd4 pulldown with Brd4 antibodies and immunoblotted with Brd4 and FLAG antibodies. (B) HEK293TT cells were transfected with glutathione S-transferase (GST)-CTD and FLAG-HPV-31 E2 Y138 mutants. FLAG pulldown with M2 gel and immunoblotted with GST and FLAG antibodies. (C) HEK293TT cells were transfected with GST-CTD and FLAG-HPV-31 E2 Y138 mutants. GST pulldown with glutathione Sepharose beads and immunoblotted with GST and FLAG antibodies. (D) HEK293TT cells were transfected with GST-BID and FLAG-HPV-31 E2 Y138 mutants. GST pulldown with glutathione Sepharose beads and immunoblotted with GST and FLAG antibodies.