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The Brd4 CTD increased binding to CDK9 in the presence of Y138E. (A) HEK293TT cells were transfected with GST-CTD and FLAG-HPV-31 E2 Y138 mutants. GST pull down with glutathione Sepharose beads and immunoblotted with GST, FLAG, and CDK9 antibodies. (B) Western blot images were quantified for CDK9 and Brd4 CTD expression. Values are expressed as means ± SEM, n = 3. CDK9 expression was normalized to Brd4 CTD expression for coimmunoprecipitation, WT = 1.
