FIG 4.

Interaction of NSs with STAT1 and STAT2. (A) HEK293T or NIH 3T3 cells were transfected with the expression plasmid for HA-tagged NSs, and co-IP assays were performed. (B) NIH 3T3 cells were transfected with the expression plasmids for HA-tagged NSs and His-tagged hSTAT2 or hamSTAT2. The protein expression levels in cell lysates (left) and in co-IP assays (right) using an anti-HA or anti-His antibody are shown. (C) Colocalization of NSs with STAT2. HEK293T, NIH 3T3, or BHK-21 cells were transfected with the expression plasmid for HA-tagged NSs and the expression plasmid for His-tagged hSTAT2, mSTAT2, or hamSTAT2, respectively. IFA was also performed with NSs, STAT2, and the nuclei shown in green, red, and blue, respectively.