FIG 5.

Determination of the region of hSTAT2 important for binding to NSs. (A) Schematic representation of the chimeric mutants of hSTAT2 and mSTAT2. (B) NIH 3T3 cells were cotransfected with the expression plasmids for HA-tagged NSs and each of the STAT2 His-tagged chimeras. Representative results from the protein expression check in cell lysates (upper) and the co-IP assays using an anti-His antibody (lower) are shown. (C) IFA was also performed with NSs, STAT2, and the nuclei shown in green, red, and blue, respectively.