TABLE 1.
List of the primers used in this study
| Name | Sequence | Remarka |
|---|---|---|
| pcDNA-NSsHA-RV-F | ATCCACCATGTCGCTGAGCAAATGCTCCAAC | For construction of pcDNA3.1/NSs-HA |
| pcDNA-NSsHA-Kpn-R | GCTTGGTACCTCAAGCGTAATCTGGAACATCGTATGGATAGACCTCCTTCGGGAGGTCACC | |
| humanSTAT2hisF | ATCCACCATGGCGCAGTGGGAAATGCTG | For construction of pcDNA3.1/hSTAT2-His |
| humanSTAT2hisR | GCTTGGTACCTCAATGGTGATGGTGATGATGACCGGTATGCATATTGAAGTCAGAAGGCATCAAGGGTCC | |
| mouseSTAT2hisF | ATCCACCATGGCGCAGTGGGAGATGTTG | For construction of pcDNA3.1/mSTAT2-His |
| mouseSTAT2hisR | GCTTGGTACCTCAATGGTGATGGTGATGATGACCGGTATGCATATTGAAGGTATCAAGAGTCCATCCCAA | |
| hamSTAT2F | ATCCACCATGGCGCAGTGGGAGACACTG | For construction of pcDNA3.1/hamSTAT2-His |
| hamSTAT2hisR | GCTTGGTACCTCAATGGTGATGGTGATGATGACCGGTATGCATATTGTCATCAGAAGGAATCAAGGGTCC | |
| mSTAT2-hDBD-veF | GTTCTTCTGCCAAGCTCCGAAAG | For construction of pHHM(#1) |
| HMM-HHM-inR | ACCGGTATGCATATTGAAGGTATC | |
| hmSTAT2DBDveF | AATATGCATACCGGTCATCATCAC | |
| mSTAT2-hDBD-inR | GCTTGGCAGAAGAACTGCTGGTTCTGAAGG | |
| hSTAT2-mDBD-inF | ACAGAGCCTTTGTAGTAGAAACCCAGCCCTG | For construction of pHMM(#2) |
| HMM-HHM-inR | ACCGGTATGCATATTGAAGGTATC | |
| hmSTAT2DBDveF | AATATGCATACCGGTCATCATCAC | |
| hSTAT2-mDBD-veR | CTACAAAGGCTCTGTGGAGCAG | |
| mSTAT2-hDBD-inF | AAAGGTCCTTTGTGGTAGAAACCCAGCCC | For construction of pMHH(#3) |
| MMH-MHH-inR | ACCGGTATGCATATTGAAGTCAG | |
| hmSTAT2DBDveF | AATATGCATACCGGTCATCATCAC | |
| mSTAT2-hDBD-veR | CCACAAAGGACCTTTGGAGCAGAC | |
| hSTAT2-mDBD-veF | GTTCTTCTCCAACCCCCCCAAG | For construction of pMMH(#4) |
| MMH-MHH-inR | ACCGGTATGCATATTGAAGTCAG | |
| hmSTAT2DBDveF | AATATGCATACCGGTCATCATCAC | |
| hSTAT2-mDBD-inR | GGGTTGGAGAAGAACTGCTGGTTCTTGGG | |
| H(1-100)MminF | CCCAGGATCCTACCCAGTTGGCTGAGATG | For construction of pH(1-100)MM(#5) |
| HMM-HHM-inR | ACCGGTATGCATATTGAAGGTATC | |
| hmSTAT2DBDveF | AATATGCATACCGGTCATCATCAC | |
| H(1-100)MMVeR | GGGTAGGATCCTGGGAAAAGGGCTGAATG | |
| H(1-221)MminF | CACTGCTAGGCCGATTAACCACCCTGG | For construction of pH(1-221)MM(#6) |
| HMM-HHM-inR | ACCGGTATGCATATTGAAGGTATC | |
| hmSTAT2DBDveF | AATATGCATACCGGTCATCATCAC | |
| H(1-221)MMVeR | ATCGGCCTAGCAGTGCTTTGGAGGCATC | |
| H222-315inF | GACTGGTTGGCCGATTAACTACCCTAATCG | For construction of pH(222-315)MM(#7) |
| hSTAT2-mDBD-veR | CTACAAAGGCTCTGTGGAGCAG | |
| hSTAT2-mDBD-inF | ACAGAGCCTTTGTAGTAGAAACCCAGCCCTG | |
| H222-315veR | ATCGGCCAACCAGTCCTTTGGAGATGTCC | |
| MH(101-315)MminF | CCCAATGGCCCTACCCAGTTGGCTGAGATG | For construction of pH(101-315)MM(#8) |
| hSTAT2-mDBD-veR | CTACAAAGGCTCTGTGGAGCAG | |
| hSTAT2-mDBD-inF | ACAGAGCCTTTGTAGTAGAAACCCAGCCCTG | |
| MH(101-315)MMveR | GGTAGGGCCATTGGGAAAGGTCTGAATATC | |
| MH(101-315)MminF | CCCAATGGCCCTACCCAGTTGGCTGAGATG | For construction of pH(101-315)HM(#9) |
| HMM-HHM-inR | ACCGGTATGCATATTGAAGGTATC | |
| hmSTAT2DBDveF | AATATGCATACCGGTCATCATCAC | |
| MH(101-315)MMveR | GGTAGGGCCATTGGGAAAGGTCTGAATATC | |
| MARV VP40 S15PF | CTTGAACCCCCCTCCTTATGCTGATCACGG | For construction of pmMARV VP40 |
| MARV VP40 S15PR | TATTGCATGTATGTGTTGTAATTGCTGGAACTGGCC | |
| MARV VP40 G79SF | GTTCCGGCATGGCTGCCTCTTGG | |
| MARV VP40 G79SR | GCTTTTGACTGTTCGCTCGTTATATGCAGATATGTC | |
| hISG56F | CCTCCTTGGGTTCGTCTACA | For real-time RT-PCR detection of human ISG56 (Ref. 13) |
| hISG56R | GGCTGATATCTGGGTGCCTA | |
| mISG56F | ACCATGGGAGAGAATGCTGAT | For real-time RT-PCR detection of mouse ISG56 (Ref. 30) |
| mISG56R | GCCAGAGGTTGTGC | |
| hOAS1realF | CATCCGCCTAGTCAAGCACTG | For real-time RT-PCR detection of human OAS1 (Ref. 13) |
| hOAS1realR | CACCACCCAAGTTTCCTGTAG | |
| mOAS1realF | GCCTGGTCACGCACTGGTA | For real-time RT-PCR detection of mouse OAS1 (Ref. 31) |
| mOAS1realR | AAGCCCTGGGCTGTGTTG | |
| hGAPDH-F | ATGGGGAAGGTGAAGGTCGG | For real-time RT-PCR detection of human GAPDH |
| hGAPDH-R | TTACTCCTTGGAGGCCATGTG | |
| mGAPDH-F | AGGTCGGTGTGAACGGATTTG | For real-time RT-PCR detection of mouse GAPDH (Ref. 32) |
| mGAPDH-R | TGTAGACCATGTAGTTGAGGTCA |
a
Ref., reference.