FIG 2.

MAPV N protein fails to inhibit IRF3 phosphorylation. HEK293T cells were cotransfected as described in the legend to Fig. 1 with a constant amount of DNA using plasmids expressing Flag-RIG-I-CARD or IRF3 and increasing amounts of plasmids expressing ANDV, NY-1V, or MAPV N proteins and the empty vector (pcDNA3.1⁺). Phospho-IRF3 (pIRF3 S-396), total IRF3, Flag-RIG-I, and N protein expression was analyzed by Western blotting. Western blot analysis of N protein, RIG-I, IRF3, and β-actin (total protein) indicates comparable protein expression levels in lysates.