TABLE 1.
HIV-1 LTR TALENs with target sites and average entropy values
| TALEN pair | Orientation | Target sequencea | Avg entropyb |
|---|---|---|---|
| TAR | Left | 5′-tGGGAGCTCTCTGGCT-3′ | 0.015 |
| Right | 3′-ACGAATTCGGAGTTATTt-5′ | 0 | |
| TATA | Left | 5′-tGCATATAAGCAGCTGCT-3′ | 0.059 |
| Right | 3′-CAGAGAGACCAATCTGGt-5′ | 0.060 | |
| RU5 | Left | 5′-tAAAGCTTGCCTTGAGTG-3′ | 0.003 |
| Right | 3′-ACGGGCAGACAACACACt-5′ | 0.034 |
a
Target sequences (left and right) for the indicated TALENs are shown. The lowercase “t” in each target sequence represents the 5′ thymine that is required for TALEN functionality but is not part of the recognized sequence.
b
Entropy scores (a measure of sequence conservation) were calculated for each nucleotide using 117 clade B LTR patient sequences from the Los Alamos sequence database, and the average was calculated for the entire TALEN recognition sequence.