Verification of miRNAs and their target genes. (A) Verification of 5 miRNAs by qRT-PCR. The expression levels of 3 up-regulated (miR-127, miR-181a, miR-191a) and 2 down-regulated (miR-1 and miR-133a) miRNAs were consistent with the sequencing data. Black is the RNA-seq data of 5 differential miRNAs (miR-127, miR-181a, miR-191a, miR-1 and miR-133a) that were negatively correlated with their target genes; white is the relative expression level of 5 differential miRNAs detected by qPCR. (B) The relative expression level of target genes (CA4, CASQ1, MBNL1, MYEF2, and IGF2) detected by qPCR. There were 3 target genes (MBNL1, CASQ1, and CA4) that were down-regulated, and 2 target genes (MYEF2 and IGF2) that were up-regulated, which were negatively correlated with their corresponding miRNA. qRT-PCR, quantitative real-time polymerase chain reaction; CA4, Carbonic anhydrase 4 ; CASQ1, calsequestrin 1; MBNL1, muscleblind-like splicing regulator 1; MYEF2, myelin expression factor 2; IGF2, insulin-like growth factor 2. The data are expressed as the mean±standard deviation (n = 3).