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. 2001 Dec 11;98(26):15050–15055. doi: 10.1073/pnas.261408198

Figure 3.

Figure 3

Protein trap lines reveal genes not predicted in the genome annotation database. In line G108, the PTT is inserted at position 44617 of the genomic scaffold AE003567, downstream of predicted gene CG10649 and upstream of CG10668. blast searches of EST databases with CG10649 and CG10668 identify regions on the 5′ and 3′ ends of EST LD29922, respectively. Besides, the 5′-most part of LD29922 matches a third prediction, CG10647, further upstream, on the adjacent scaffold AE003566. Therefore, segments of all three predictions (CG10647, CG10649, and CG10668) are part of a single gene, which spans ≈120 kb. The insertion in line G108 reveals the expression of this gene: 3′ cDNA sequences fused to GFP match sequences of CG10668. Predicted genes are in blue, sequenced parts of the EST are in red, and the region found to be fused with GFP in the 3′ rapid amplification of cDNA ends experiment is in green.