Table 1.
Transposition rate and frequency of GFP+ insertions
| Construct | Mutator line | Sb-w+/Sb tot | Tranposition efficiency (%) | Green frequency |
|---|---|---|---|---|
| P-GA | GAIII-1b | 41/252 | 16.3 | 1/1,540 |
| P-GB | GBIII-3a | 5/144 | 3.5 | nd |
| GBIII-3b | 24/246 | 9.6 | 1/1,785 | |
| GBIII-5 | 5/183 | 2.7 | nd | |
| P-GC | GCIII-1 | 2/228 | 0.9 | nd |
| GCIII-3 | 4/294 | 1.4 | nd | |
| GCIII-4a | 2/104 | 1.9 | nd | |
| GCIII-4b | 41/227 | 18.1 | 1/1,600 | |
| GCIII-5 | 2/124 | 1.6 | nd |
To select mutator lines with high transposition frequency, jumpstart males with the PTT, w+/Delta2-3Sb genotype were mated with yw virgin females (see Fig. 1c). The transposition frequency was scored among the Sb progeny as the percentage of individuals showing a variegated eye phenotype. Sb flies have inherited the Delta2-3Sb chromosome III, and not the jumpstart chromosome III, from their father. The presence of the w+ marker in Sb individuals can therefore only result from a transposition of the PTT-w+ from its original localization on the jumpstart chromosome to a new position. The green frequency is the number of GFP-positive insertions divided by the total number of larvae screened. For each mutator line, the figures were calculated in the beginning of the screen out of a total number of approximately 40,000 larvae. nd, not determined.