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. 2019 Mar 13;6(9):1801361. doi: 10.1002/advs.201801361

Figure 2.

Figure 2

High‐throughput, multiplexed, sequential secretion analysis from the same single cells. A) Schematic illustration of the procedure for tracking the secretion from the same single cells at different time points. Single‐cell secretions were first profiled at the basal state, then LPS was added to induce macrophage responses to TLR4 activation. The time course of LPS‐induced activation is measured sequentially within different time windows; B) Characterization of single cell retention efficiency with human macrophage cells after a new antibody glass slide was changed to PDMS microchamer array (n = 5); C) Representative single cell and its secretion pattern of 10 proteins from the same single cell at 4 time points, before and after LPS treatment; D) Line graphs showing the dynamic change of different secreted proteins (CXCL8, IL‐6, CCL2, and TNF) from 1752 single cells, which were classified into four patterns based on their protein secretion dynamics: on–off, off–on, all‐on, or others.