FIG 8.

OX-yedQ strain was more resistant to H₂O₂ in P. syringae. (A) The expression of sodA, sodB, and sodC based on RNA-seq. The relative gene expression level in the OX-yhjH strain was set to 1, and the other values were adjusted accordingly. (B) OD₆₀₀ of bacterial culture in 2 mM H₂O₂ in KB liquid medium after 48 h of cultivation. The OD₆₀₀ was measured for every 12 h. **, significantly different between OX-yedQ and OX-yhjH strains, P < 0.01; ***, significantly different between OX-yedQ and OX-yhjH strains, P < 0.001. All experiments were performed in triplicate. Error bars indicate standard deviations. (C) Hydrogen peroxide resistance of colony assay. Bacterial culture was adjusted to an OD₆₀₀ of ∼1.0. A KB agar plate was added 0.5 mM H₂O₂ and was dotted with 10 μl of 10-fold-diluted bacteria. Photos were taken after 3 days of cultivation at 28°C. Three H₂O₂ plates were used for two strains, and the experiment was repeated with two independent bacterial cultures. Ctrl, control.