FIG 6.

Plate-based bacteriocin assay. Washed cells of each clinical commensal were mixed with UA159 at a 1:1 ratio based on optical density and stabbed onto TY agar plates containing 20 mM Glc, GlcN, or GlcNAc. The plates were incubated overnight, overlaid with soft agar containing S. sanguinis SK150, and then incubated for another 24 h. The experiments were carried out in both aerobic and anaerobic environments.