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. 2019 May 2;85(10):e02335-18. doi: 10.1128/AEM.02335-18

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Copyright © 2019 Alexander et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — L. reuteri-mediated leptin production. (a) Western blotting results for intracellularly accumulated leptin indicated that leptin-3×FLAG is produced at the expected size, while the majority of secreted leptin is incorrectly cleaved. Lane I, LR/pLeptin-3×FLAG (19 kDa); lane SP, secreted leptin LR/pSP-Leptin-3×FLAG (23 kDa). (b) ELISA confirmed leptin production by LR/pLeptin. Ctl, LR/pCtl; nd, not detected. The results shown are averages from three independent experiments ± standard error, normalized per 1 mg of cell pellet dry weight.