TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmida	Characteristicsb	Source or referencec
Strains
E. coli EC1000	Derivative of E. coli MC1000 in which repA is integrated in the chromosome	85
L. reuteri
VPL1014	Human breast milk isolate	Lab stock
VPL4090	Mutant lacking both active phages and attB sites, lytic host	55
VPL4121	Mutant lacking both active phages, restored attB sites, LRΔΦ1ΔΦ2	55
VPL4224	Rifr mutant generated with oVPL236 for mutation in rpoB gene (H488R)	55
VPL4243	Derived from VPL4224, mutations introduced in thyA (Y38*, Q39S, M40L) with oVPL1670	This work
L. rhamnosus
ATCC 53103	Human fecal isolate	ATCC
VPL4141	Rifr natural mutant isolated from MRS medium-Rif25 plate	This work
L. casei BFLM218	Human fecal isolate	35
L. fermentum ATCC 14931	Fermented beet isolate	ATCC
L. plantarum
ATCC BAA-793	Human saliva isolate	ATCC
VPL4142	Rifr natural mutant isolated from MRS medium-Rif25 plate	This work
L. salivarius CCUG 47825	Human blood isolate	CCUG (86)
L. gasseri ATCC 33323	Human intestinal isolate	ATCC
Lactococcus lactis subsp. cremoris
NZ9000	Dairy starter, derivative of MG1363, pepN::nisRK	87
VPL4005	Rifr mutant generated with oVPL234 for mutation in rpoB gene (H486N)	This work
L. jensenii ATCC 25258	Human vaginal isolate	ATCC
L. acidophilus ATCC 4356	Human isolate	ATCC
Plasmids
pVPL2042	Emr, pNZ8048 derivative; the Cmr marker was replaced by an Emr marker	Lab stock
pVPL3583	pJP028 vector control (pCtl)	This work
pVPL3585	pJP028 derivative, pNZ-EFTu-SP-Leptin	This work
pVPL3752	pJP028 derivative, pJP-EFTu-SP-Leptin-3×FLAG	This work
pVPL3791	pJP028 derivative lacking the signal peptide, pNZ-EFTu-Leptin	This work
pVPL3795	pJP028 derivative, pJP-EFTu-Leptin-3×FLAG	This work
pVPL31131	pJP028 derivative, pJP-EFTu-Leptin-ThyA	This work
pVPL31134	pJP028 derivative, pCtl-ThyA	This work

^aVPL, van Pijkeren Laboratory strain identification number; pVPL, van Pijkeren Laboratory plasmid identification number.

^brepA, gene for replication initiation protein; attB, phage insertion site; Em^r, erythromycin resistant; Cm^r, chloramphenicol resistant; Rif^r, rifampin resistant; Rif25, rifampin at 25 μg/ml; rpoB, gene encoding β subunit of RNA polymerase, homolog of LAR_1402 in L. reuteri JCM1112; thyA, gene encoding thymidylate synthase, homolog of LAR_0739 in L. reuteri JCM1112. An asterisk (*) indicates a stop codon. The locus tags listed can be found at https://www.ncbi.nlm.nih.gov.

^cATCC, American Type Culture Collection.