FIG 11.

Comparison of gene fitness values from a chemogenomic profiling study (41). Values along the x and y axes are gene fitness values for the growth conditions indicated. Gene fitness values reflect the change in population frequency for a given Tn mutant from the time of inoculation until the end of the experiment (6 to 8 generations). Negative values indicate that a Tn insertion lowered strain fitness. Positive values indicate that a Tn insertion increased strain fitness. More information on the conditions and calculations used to determine gene fitness values is available elsewhere (41, 44). Each gray dot represents the gene fitness value for a given ZM4 gene. Graphs compare gene fitness values in a minimal aerobic medium (rep 1) with those for a replicate under an identical growth condition (control; rep 2) (A) or with those in a minimal anaerobic medium (B) or a rich aerobic medium (C).