FIG 6.

NADH DH mutants show improved growth trends and a higher ethanol yield in rich PYG medium. Shown are representative growth curves (A), final OD values (B), and major fermentation product yields (C) for wild-type ZM4 (WT) and NADH DH mutants (Ndh::Tn) in PYG medium. EV, empty vector pSRKTc; comp, complementation vector pSRKTc-Ndh. (B) Bars with different letters are significantly different (P < 0.05) as determined by one-way analysis of variance with Sidak’s multiple-comparison posttest. Error bars, standard deviations (n = 2). Trends similar to those shown in panel A were observed for at least one other biological replicate.