Overview of the pVP-R2lc01 and pVP-R2lc02 plasmids and their predicted PKS clusters. (A) The 23.8-kb pVP-R2lc01 plasmid contains 25 ORFs (indicated by arrows), including the fun cluster (ORFs 1 to 12). (B) The 21.4-kb pVP-R2lc02 plasmid contains 28 ORFs and carries the pks cluster (ORFs 1 to 15). Differences in GC contents of each gene (compared to the chromosomal GC content) are represented with different colors, as shown on the right. (C) The fun cluster (top) spans 13.4 kb containing 12 ORFs, and the pks cluster (bottom) spans 11.3 kb containing 15 ORFs. Transport-related, additional biosynthetic, core biosynthetic, regulatory, and other genes are represented (for gene annotations, see Tables 2 and 3).