FIG 6.

PKS is responsible for AhR activation. (A) R2lc∆pks does not absorb at 395 to 410 nm, in the region where R2lc and R2lc∆fun exhibited maximum absorbance. (B) The fun cluster does not drive AhR activation of L. reuteri R2lc. (C) Inactivation of the pks cluster significantly reduces the ability to activate AhR. (D) Extracts used for AhR activation assays do not impact cell viability. Data shown in bar graphs are averages of results from at least 3 biological replicates, and error bars represent standard deviations. A one-sample t test was used to compare strains and the negative control, and Student’s t test was used to compare wild-type and mutant strains (a P value of <0.05 was considered significant). ns, not significant; WT, wild type.