TABLE 5.
Plasmids used in this studya
| Plasmid | Characteristic(s) | Reference |
|---|---|---|
| pVPL3002 | pORI19 harboring L. reuteri-derived ddlF258Y | 29 |
| pVPL3669 | Emr; derivative of vector pVPL3002 in which the L. reuteri R2lc Δfun deletion cassette was cloned into the MCS | This study |
| pVPL3805 | Emr; derivative of vector pVPL3002 in which the L. reuteri R2lc Δpks deletion cassette was cloned into the MCS | This study |
| pVPL31010 | Emr; derivative of vector pVPL3002 in which the L. reuteri 2010 Δpks deletion cassette was cloned into the MCS | This study |
| pVPL31041 | Emr; derivative of vector pVPL3002 in which the L. reuteri R2lc Δpks::Cm insertion cassette was cloned into the MCS | This study |
a
pVPL, van Pijkeren Laboratory plasmid identification number; MCS, multiple-cloning site; ddlF258Y, a derivative of ddl in which mutations are made yielding the amino acid change phenylalanine to tyrosine at position 258 in the d-Ala-d-Ala (Ddl) protein.