Fig. 4.

Role of Akt2 in migration induced by EVs from MDA-MB-231 cells treated with LA in MCF10A cells. Panel a. Akt2 expression of MCF10A cells transfected with Akt2-specific or scramble siRNAs was analyzed by Western blotting with anti-Akt2 Ab. One control of actin was included. Graph is the mean ± S.D. and is expressed as fold of Akt2 under Ctrl. Panel b. Migration assays were performed with MCF10A cells transfected with Akt2 siRNAs or scramble siRNAs and treated with EVs from MDA-MB-231 cells untreated or treated with LA. Graph is the mean ± S.D. and is expressed as fold of migrated cells above Ctrl EVs. Asterisks indicate comparisons made to Ctrl, scramble siRNA and Ctrl EVs. *P < 0.05, **P < 0.01