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. 2018 Nov 11;13(2):225–233. doi: 10.1007/s12079-018-0495-x

Fig. 5. TGF-β1 mediated mRNA expression of GAG genes (A) CHST11 and (B) CHSY1 is mediated via Nox dependent pathways.

Fig. 5

VSMCs were pre-incubated with the following inhibitors: Nox inhibitors DPI (20 μM) and apocynin (20 μM) for 2 h and TGFBR1 antagonist, SB431542 (10 μM) for 30 min before being treated with TGF-β1 (2 ng/ml) for 6 h. Total RNA was harvested and the mRNA of a CHST11 and b CHSY1 were analyzed using qRT-PCR. GAPDH was used as a house keeping gene. Results are expressed as mean ± SEM from three independent experiments and statistical significance was determined by One-way ANOVA followed by least significant difference post-hoc analysis. **p < 0.01 compared with untreated control, #p < 0.05 and ##p < 0.01 compared with TGF-β1