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. 2018 Nov 15;13(2):209–224. doi: 10.1007/s12079-018-0493-z

Fig. 7. Myo10 up-regulation upon Nef expression is cell type independent and happens in human MDM during HIV-1 infection.

Fig. 7

Western blot analyses of Myo10 expression in (a) mouse neuronal CAD cells and (b) human epithelial HeLa cells transfected with GFP-vector (control) and Nef-GFP are shown. Calnexin was used as loading controls. Blots are representative of 3 independent experiments, P value = 0.005 (*). In both cell types, Myo10 expression increases upon Nef-GFP expression. Human MDM were infected with reverse transcriptase-normalized VSV-G pseudotyped virus stocks (WT = pNL4–3 clone) or (DelNef = pNL4–3delNef clone) at 5, 10, and 20 cpm of reverse transcriptase activity/cell. (c) A representative Western blot of the WT or DelNef infected cells from 3 independent infections is shown along with (d) Densitometry representation of the relative expression levels of Myo10 normalized to the amount of tubulin (loading control), with a P value <0.01 (**) or < 0.05 (*)