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. 2019 Apr 26;10:453. doi: 10.3389/fphar.2019.00453

FIGURE 2.

FIGURE 2

Chronic post-ischemia pain (CPIP) increased TRPV1 expression in rat dorsal root ganglion neurons. (A) Representative immunofluorescence images indicating TRPV1 antibody staining of DRGs from Sham and CPIP-7 d group rats. Ipsilateral L4-6 DRGs were collected 7 days post Sham or CPIP model establishment. Areas staining positive for TRPV1 are shown in green. DRGs were co-stained with NeuN antibody (red) to identify DRG neurons. Scale bar indicates 50 μm. (B) Summary of the % of TRPV1 positively stained neurons (TRPV1+) from each observation field. The total number of DRG neurons per field was calculated based upon positive NeuN (NeuN+) staining. (C) Summary of the normalized % increase in fluorescence intensity of TRPV1 staining in the observation field as in A. The value was normalized to Sham group. (D) Size distribution of TRPV1+ neurons in DRGs of Sham and CPIP-7 d group rats. Five observation fields from three rats were included in each group. (E,F) TRPV1 protein expression in DRGs (E) and hind paw skin (F) of Sham and CPIP group rats measured with Western blot. Upper panel indicates representative images of TRPV1 and GAPDH protein expression from Sham and CPIP-7 d and 14 d group rats. Lower panel indicates summarized TRPV1 expression normalized to GAPDH. n = 5 rats/group. ∗p < 0.05 vs. Sham group. Student’s t-test or one-way ANOVA followed by Tukey post hoc test was used for statistical analysis.