Fig. 6.

10C4–8E7 MAb specifically decrease the amount of NA of Len/17-MDV in selective passage reassortants pool. (A) Pyrograms of NA gene of clones from selective passage of co-infection between Len/17-MDV (H2N2) and A/Texas/50/2012 (H3N2) in the presence of antibody to HA subtype 2 of A/Japan/305/1957 (H2N2) (Mix 1, representative) or in combination with 10C4–8E7 mAb to N2-NA of Len/17-MDV (Mix 2, representative) and the reference pyrograms of Len/17-MDV and A/Texas/50/2012. The signature nucleotides/peaks of desired A/Texas/50/2012 NA gene in the reassortant are pointed by green arrows, undesired Len/17-MDV NA gene – in red. The nucleotides dispensation order for the sequencing is shown at the bottom. (B) The quantitative assessment of picks height for NA gene was done using “Peak Height Report” of PyroMark Q96 2.5.8 software on pyrograms of RT-PCR products of selective passage RNA (three for each condition). The height of signature peaks of Len/17-MDV NA (C1, T4, T9– red) and the signature peaks of A/Texas/50/2012 NA (C3, T7 - green) was assessed and the average values ± SD for each nucleotide/signature pick are shown.