Figure 2.

Effects of CLDN8 on CRC cell proliferation migration and invasion.

Notes: (A, B) Western blot analysis confirmed the successful transfection and stable establishment in both SW480 and HT-29 cells. (C) The results of CCK-8 assay revealed that CLDN8 overexpression promoted the proliferation of SW480 and HT-29 cells, while CLDN8 knockdown significantly decreased the proliferation rate in a time-dependent manner. (D, E) The results of Transwell invasion assay showed that upregulation of CLDN8 expression in SW480 and HT-29 cells significantly enhanced cell invasion, which was inhibited by CLDN8 knockdown. (F–H) Wound healing assay showed that CLDN8 overexpression promoted SW480 and HT-29 cells migration, whereas downregulation of CLDN8 markedly reduced cell migration. The meaning of each group name: CLDN8-NC, the negative control group of CLDN8 overexpression; CLDN8, overexpression of CLDN8; Knock-NC, the negative control group of TROP2 knockdown; Knock, knockdown of CLDN8. *P<0.05 when compared with CLDN8-NC. ; **P<0.01 when compared with CLDN8-NC. Bar = 100μm.

Abbreviations: CCK-8, Cell Counting Kit-8; CRC, colorectal cancer.