FIGURE 1 .

Effect of in vitro supplementation with α-T3 (A), γ-T3 (B), and δ-T3 (C) on lymphocyte proliferation of young and old mice. Splenocytes from C57BL/6 mice fed a nonpurified mouse diet were incubated with various concentrations of α-, γ-, or δ-T3 for 4 h and stimulated by with 1.5 mg/L Con A for 72 h. Values are mean ± SEM, n = 6. Three-way ANOVA revealed a significant age effect and an interaction of age × concentration × T3 type. For each variable, labeled means without a common letter differ, P < 0.05. *The difference from its control (0) is greater than that for γ-T3 or δ-T3 at the same concentration (P < 0.01). #The difference from its control (0) is less than that for α-T3 (P< 0.001) and greater than that for δ-T3 (P < 0.05) at the same concentration. The difference from its control (0) is less than that for α-T3 (P < 0.01) and greater than that in δ-T3 (P < 0.01) at the same concentration.