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. 2019 May 2;16:56. doi: 10.1186/s12985-019-1167-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Phylogenetic analysis of PARV4-related viruses based on NS1(A), VP1(B) and VP2(C). The phylogenetic tree was constructed by using maximum likelihood (ML) with MEGA 7.01 software (http://www.megasoftware.net). Bootstrap values were calculated with 1000 replicates. The number on each branch indicates bootstrap values. The strains identified in the phylogenetic tree are indicated by GenBank accession numbers and breeds and strains. The seven P-PARV4 strains and eight O-PARV4 strains newly identified in the present study are indicated by “▲” and “●”, respectively. P-PARV4, O-PARV4 and B-PARV4 are shown in red, blue and green, respectively. Scale bar indicates nucleotide substitutions per site