Fig. 2.

Expression analysis of OsS40 genes during natural senescence of flag leaves. a Phenotype of rice flag leaves collected at different late development stages (90DAG, 97DAG, 104DAG, 111DAG, 118DAG). DAG, Days after germination. b Development dependent alteration of chlorophyll content in rice flag leaves. c qRT-PCR analyses of the six differentially expressed OsS40 genes along with senescence marker gene, OSH36. Transcript levels are expressed relative to that of rice actin in each sample, and values are mean ± S.D. Student t-test was used to generate P value. *P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001