Fig. 4.

Expression analysis of OsS40 genes in response to dark treatment. a Phenotype of detached rice leaves after darkness treatment. Detached leaves from 4-week-old rice seedlings were incubated in the deionized water in darkness for 2 days (D). As a control, the detached leaves were incubated with water at the same time in a light/dark regime (L). b qRT-PCR analysis of the four differentially expressed OsS40 genes along with senescence marker genes, OsNAP, OSH36 and Os157, after darkness treatment. Transcript levels are expressed relative to that of rice actin in each sample, and values are mean ± S.D. Student t-test was used to generate P value. *P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001