Fig. 5.

Expression analysis of OsS40 genes under different hormone treatments. a Phenotypes of detached leaves from four weeks old rice plants under different hormone treatments. Photos of detached leaves floating on 200 μM concentration of ABA, SA, MeJa or IAA solution were taken at 0 h, 24 h, 48 h and 72 h. b Quantitative real-time PCR analyses of the eight differentially expressed OsS40 genes along with senescence marker genes, OSNAP, SGR, NYC1, NYC3 and OS157, upon ABA, SA, MeJA or IAA treatment. Transcript levels are expressed relative to that of rice actin in each sample, and values are mean ± S.D. Student t-test was used to generate P value. *P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001