Table 2.

Correlation of plasmid stabilization and ParA-Gfp oscillation

Plasmid*	par2 components†	LF values‡	Folds of stabilization	Oscillation of ParA-Gfp
pMH82	None	2.0  × 10−2	1.0	No ParA-Gfp
pGE2K	parC1 parA parB parC2	1.3  × 10−4	150	No ParA-Gfp
pGE202K	parC1 ΔparA parB parC2	2.0  × 10−2	1.0	No ParA-Gfp
pGE236	parC1 ΔparA parB parC2 parA∷gfp	2.7  × 10−4	75	+
pGE236G10V	parC1 ΔparA parB parC2 parA(G10V)∷gfp	2.0  × 10−2	1.0	−
pGE236K14Q	parC1 ΔparA parB parC2 parA(K14Q)∷gfp	3.2  × 10−2	0.6	−
pGE233	parC1 parA parB parC2 parA∷gfp	5.0  × 10−4	40	+
pGE230	parA∷gfp	1.7  × 10−2	1.2	−

^*All plasmids were derived from pMH82, a par⁻ R1 test vector. 

^† Δ indicates a 213-bp in-frame deletion in parA. 

^‡ LF values are defined in the legend to Fig. 1. Plasmid-containing cells of strain KG22 (lacI 

^q) were grown in LB medium at 30°C without IPTG. Culture dilutions were plated on LA plates containing X-gal (40 μg/ml) to determine the frequency of plasmid-containing cells. Numbers are in all cases averages of at least three independent experiments.