Fig. 6.

ATPR induces the accumulation of intracellular ROS, leading to cell cycle arrest at G0/G1 and differentiation. a NB4 cells were treated with ATPR (10⁻⁶ M) for different durations (0–72 h). Intracellular ROS levels were assessed by flow cytometry. b Intracellular ROS levels were assessed by immunofluorescence staining after treatment with 10⁻⁶ M ATPR for 72 h. c–e NB4 cells were pretreated with NAC and Tiron for 1 h and then treated with ATPR (10⁻⁶ M) for 72 h. The intracellular ROS levels were measured by flow cytometry, and CD11b and CD14 expression was measured by flow cytometry. f NB4 cells were pretreated with NAC for 1 h and then treated with ATPR (10⁻⁶ M) for 72 h. CD11b levels were analyzed by western blot. *p < 0.05, **p < 0.01 versus control group; ^#p < 0.05, ^##p < 0.01 versus ATPR group