Fig. 3.

Copy number of centromeric K111 in different stages of CTCL. Centromeric K111 was quantitated in the DNA of PBLs by qPCR using a LNA primer that is highly specific for K111 gag. The copy number was determined in the same run using a standard curve generated by dilution of DNA chosen from a CTCL patient who had the highest levels of K111 measured in our earliest assays. Note that one CTCL patient had a relatively low copy number while all the rest of the patients have either high copy numbers of K111 or have a −/−K111 genotype. Note that the DNA from the PBLs of a patient who had a bone marrow transplant (BMT) had a high copy number of K111 while her buccal swab (Fig. 1c) is −/− K111. p values were calculated using a t-test. Statistical significance p < 0.05 (*), p < 0.01 (**)