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. 2019 May 2;16:27. doi: 10.1186/s12986-019-0353-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The effects of myricetin on muscle fiber-type composition in L6 myotubes. Cell viability (a) was measured using CCK-8 assay. The mRNA expressions of slow-twitch myosin Myh7 (b), fast-twitch myosin Myh1 (c), Myh4 (d), and slow-twitch fiber biomarker myoglobin (e) were determined by real-time PCR. Protein levels of slow-twitch myosin Myh7 and slow-twitch fiber biomarkers (f) in L6 myotubes were measured by immunoblotting. Data are presented as mean ± SD. *P<0.05, **P<0.01, ***P<0.001 compared with control