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. 2019 Jan 29;21(3):309–318. doi: 10.4103/aja.aja_103_18

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Copyright: © The Author(s)(2019)

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Expression profile of PLZF in cross section and whole mount IHC. PLZF was immune-stained strongly in undifferentiated A spermatogonia throughout the seminiferous cycle. (a) A₄ was PLZF^LOW in stage I, (b) In was PLZF^NEG in stage III, (c) B was PLZF^NEG in stage V, (d) A₁ was PLZF^HIGH in stage VII, (e) A₂ was PLZF^LOW in stage X, (f) A₃ was PLZF^LOW in stage XII in cross section and whole mount IHC. A_un: black arrows; A₁: red arrows; A₂: green arrows; A₃: yellow arrows; A₄: blue arrows; In: grey arrows; B: cyan arrows; preleptotene: black asterisks; leptotene: red asterisks; zygotene: green asterisks; early pachytene: yellow asterisks; mid-pachytene: blue asterisks; late pachytene: grey asterisks; diplotene: pink asterisks; meiotic division: cyan asterisks; Sertoli cells: black arrowheads; round spermatid: black triangles; elongating spermatid: red triangles. Scale bar=25 μm. PLZF: promyelocytic leukemia zinc finger; IHC: immunohistochemistry.