Figure 3.

Expression profile of GFRA1 in cross section and whole mount IHC. GFRA1 immuno-staining was detected mostly on the cytomembrane of A_s, A_pr and A_al4 undifferentiated spermatogonia clones across the seminiferous cycle. No GFRA1 staining was detected in (a) A₄ in stage I, (b) In in stage III, (c) B in stage V, (d) A₁ in stage VII, (e) A₂ in stage X and (f) A₃ in stage XII in cross section and whole mount IHC. Indicators of multiple germ cell types were the same as in figure 1. Scale bar=25 μm.