Table 1.
LC‐MS/MS settings and conditions
| Liquid chromatography gradient conditions | |||
|---|---|---|---|
| Time (min) | Flow rate (mL/min) | Solvent A%a | Solvent B%a |
| 0 | 0.15 | 70 | 30 |
| 2 | 0.15 | 30 | 70 |
| 6.5 | 0.15 | 30 | 70 |
| 7.5 | 0.15 | 70 | 30 |
| 10 | 0.15 | 70 | 30 |
| Mass spectrometer settings | |
|---|---|
| Curtain gas | 15 psi |
| Ion spray voltage | −4500 V |
| Dissolvation temperature | 600°C |
| GS1 and GS2 | 20 and 40 psi, respectively |
| Declustering potential | −110 V |
| Collision energy (CE) for different MRM transitions | |
| Succinyl‐CoA | 866.2 > 408 (CE −55 eV) |
| D4‐Succinyl‐CoA | 870.2 > 408 (CE −55 eV) |
| Malonyl‐CoA | 852.2 > 808 (CE −40 eV) |
a
Solvent A, 10 mM ammonium formate in acetonitrile/water (90/10, vol/vol); Solvent B, 10 mM ammonium formate in acetonitrile/water (20/80, vol/vol).