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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Cell Physiol. 2018 Mar 7;233(8):5736–5746. doi: 10.1002/jcp.26281

Figure 2.

Figure 2.

A. Adenosine stimulates Rac1 activity in HPAEC. To measure Rac1 activity in adenosine- treated HPAEC, cells were harvested at different time points (5, 10, 15, and 20 min) and Rac1 G-LISA Activation Assay Kit was used according to the manufacturer’s instructions. Cells were serum starved for 2 hours before adenosine treatment and Rac1 activity was measured, n=4; mean +/− SEM * p<0.05 compared to 0 min control. B and C. Adenosine decreases MLC, but not MYPT1 phosphorylation. HPAEC were treated with adenosine at different time points. Cells were harvested and western blots were performed to detect changes in phosphorylated MLC (Thr18/Ser19) and MYPT1 (Thr 853) levels (panels B and C, respectively). Actin was used as a loading control. Bar graphs show the changes of phospho- MYPT1 and diphospho-MLC quantified with scanning densitometry. Results are expressed as Mean and SEM (n=4 experiments). *<0.05 vs. control.