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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Cell Physiol. 2018 Mar 7;233(8):5736–5746. doi: 10.1002/jcp.26281

Figure 8. Tiam1 depletion has no effect on adenosine-induced increase in TER and Rac1 activation in pulmonary endothelial cells.

Figure 8.

A. HPAEC were treated with Tiam1 specific siRNA or non-specific siRNA and then challenged with 50 μM adenosine in TER measurement assay. Depletion of Tiam1 did not affect adenosine-induced endothelial barrier enhancement. Arrow indicates the time point when adenosine was added. n=4; mean +/− SEM; B. Effect of adenosine on Rac1 activation in Tiam1 depleted cells. HPAEC were transfected with Tiam1 specific siRNA or non-specific siRNA and then 72 hours later challenged with 50 μM adenosine at different time points in Rac1 activity assay. Cells were serum starved for 2 hours before adenosine treatment and Rac1 activity was measured according to the manufacturer’s protocol. n=4; mean +/− SEM; **P < 0.01 vs. Non-siRNA/ 0 min; #p < 0.05 vs. Tiam1-siRNA/0 min, ##p < 0.01 vs. Tiam1-siRNA/0 min.