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. 2019 Apr 26;9:314. doi: 10.3389/fonc.2019.00314

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Copyright © 2019 Nourmohammadi, Aung, Cui, Pei, De Ieso, Harata-Lee, Qu, Adelson and Yool.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of CKI and fractions on cell invasiveness in eight cell lines. Migration efficiencies of cells across an extracellular matrix barrier after treatment with CKI, minor (MN), major (MJ), or combined (MN+MJ) fractions were measured by transwell invasion assays. (A) Images show stained cells that had passed through the filter to reach the opposite side of the transwell membrane. CKI, MN and MJ were applied at doses equal to those present in 2 mg/ml CKI; Ctrl is vehicle control. (B) Compiled results are shown in histograms; n-values are in italics in corresponding figure key. Statistically significant differences compared to vehicle control are indicated as *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001; ns, not significant.