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. 2019 Apr 26;9:314. doi: 10.3389/fonc.2019.00314

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Copyright © 2019 Nourmohammadi, Aung, Cui, Pei, De Ieso, Harata-Lee, Qu, Adelson and Yool.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analyses of four proteins: CTNNB1, AKT (1–3), CCND1, and ACTB compared in three cell lines with and without CKI-based treatments. The levels of protein expression for four genes predicted by transcriptomic analysis to be significantly down-regulated were evaluated by FACS in three cell lines (A) MDA-MB-231, (B) HEK-293, and (C) HFF. Statistically significant differences compared to vehicle controls were analyzed using one-way ANOVA and post hoc tests and are shown as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and not significant (ns).