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NK cells function is altered in the tumor compared to adjacent tissue. Isolated cells from either the tumor or the matching adjacent tissue were cultured overnight at 37°C. They were then stimulated with PMA/Ionomycin for 6 h and stained for intracellular cytokines. (A) We compared the percentage of intracellular expression of IFN-γ and TNF-α, or, (B), Granzyme-B (GrzB) and CD107 in NK cells. Each dot/line represents one patient n = 5.
