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. 2019 Apr 23;12:3087–3098. doi: 10.2147/OTT.S200261

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© 2019 Huang et al.

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RARα suppresses MMP2 transcription. (A) QPCR to determine the mRNA levels of MMPs in RARα-knockdown RKO and RARα-overexpressed HT29and control cells. (B) The enzymatic activity of MMP2 were assessed by gel zymography assays. (C) MMP2 promoter activity was increased by overexpressing RARα and was decreased by the knockdown in 293T line. Normalization of firefly luciferase was done with reference to renilla luciferase. (D) Luciferase activity of various pGL3-MMP2 vectors (wild-type or deleted) whether exogenous RARα was present or absent in 293T cells. (C and D) A representative assay of independent and triplicate assays is displayed. *P<0.05, **P<0.01, ***P<0.001.