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. 2018 Feb 3;29(3):963–977. doi: 10.1093/cercor/bhy004

Figure 1.

Figure 1.

Generation of Nex-CreERT2:NrCAM; tdT conditional mutant mice. (A) PCR genotyping showing diagnostic fragments for WT (532 bp), NrCAM F/+ (532 and 728 bp), and NrCAM F/F (728 bp) mice. (B) tdTomato labeling of V1 neurons in NrCAM F/+ and NrCAM F/F mice (P21) induced at P10–13, and lack of labeling in uninduced mice (bar=100 μm, layers 1–6). (C) NrCAM immunoblot from cortical lysates of NrCAM F/+ or F/F mice (P21). Histogram shows the relative pixel densities of the blot (mean ± SEM, n = 3, *P < 0.05, t test). (D) NrCAM in pyramidal neurons marked by tdTomato in NrCAM F/+ but not in NrCAM F/F mice (V1, layer 4, P21 and P80 (bar = 50 μm). (E) tdTomato+ neurons in NrCAM F/+ mice express vGlut1 but not GAD67 (bar = 50 μm). (F) tdTomato labeling of MFC neurons (Cg1) in NrCAM F/F mice (P21) after early postnatal induction at P10-13, and lack of labeling in uninduced F/F mice (bar=100 μm, layers 1–6). (G) NrCAM in pyramidal neurons of MFC colocalizes with tdTomato in NrCAM F/+ but not in NrCAM F/F at P21 and P80 (bar = 50 μm).