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. Author manuscript; available in PMC: 2019 May 3.
Published in final edited form as: Methods Enzymol. 2019 Feb 21;618:49–72. doi: 10.1016/bs.mie.2018.12.020

Fig. 2.

Fig. 2

The levels of ubiquitin were analyzed by Western blotting (polyclonal antibody from Santa Cruz, SC-9133) in the indicated strains. The GPDpr-Ub construct encoding three tandem wild-type ubiquitins was integrated at UBI4.